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Cairn Optoscan Monochromator Systems
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The Optoscan monochromator is a high speed, high resolution wavelength changer, incorporating nanometer precision of both centre wavelength and bandwidth settings. Wavelength and bandwidth changes are achieved in milliseconds, with fast galvanometers driving both the grating and slit positions to give full dynamic control of optical throughput. The Optoscan was designed with biological fluorescence measurements in mind, and when used together with our signal processing modules it forms the heart of a powerful and flexible fluorescence detection system. It can also easily be incorporated into commercial imaging systems or custom controlled for bespoke applications. The monochromator has evolved into a truly versatile lab tool, so if your application requires fast, flexible and automated illumination control then the Cairn Optoscan may well be your instrument of choice.
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Main Features :
- Millisecond control of centre wavelength.
- Nanometer wavelength precision over broad spectrum
- Dynamic bandwidth control.
- Microsecond timing precision
- High throughput f/2 optics
- External iris diaphragm for intensity control
- Fast electronic shuttering facility.
- Compact design.
- Parallel, serial and DAC control options
- Wavelength selection ready signal for external interfacing.
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Further Information :
System Configuration
A basic Optoscan monochromator system consists of four main components: The monochromator unit itself; choice of Optoscan controller unit; an arc lamp light source; and a power supply for the arc lamp. The monochromator is designed to be used with the Cairn Optosource, and delivers its output into a light guide for coupling to a microscope. The monochromator is both an instrument in its own right and an integral part of the Cairn product range, from which complete photometric and imaging systems can be assembled. Not only does it have a number of powerful features but we also provide full hardware and software support to enable you to use these features effectively. The prefered method of controlling the monochromator will depend to a large extent on the environment in which it is to be used, and thus there are a range of options for controlling it. These fall into three catagories, a rack mounted controller with support for our photometry modules; a standalone controller without photometry module support; and lastly a computer interface card for direct computer control. Each of these is detailed in the operation and control section.
In addition to the controller options, the monochromator unit can be supplied in a number of configurations. The recommended choice is always the fully automated version with fast galvanometer driven grating and slits to provide wavelength changes in milliseconds and nanometer control over bandwidth. However, for users on a restricted budget we can provide versions of the monochromator with manually controlled slits If required we can also provide a fixed input slit, but it is important to realise this type of arrangement does not provide a fixed bandwidth, as the light diffraction angle off the grating is wavlength dependent. As an example, an exit slit width giving a 10nm bandwidth at 300nm will give a bandwidth of around 12.5nm at 400nm. See the monochromator technical notes for more information on this.
Interfacing with external equipment
i) Optical coupling
The monochromator unit is normally supplied with a liquid light guide output, which can be connected to the epifluorescence input of any standard fluorescence microscope by using the appropriate light guide coupling. Alternatively it can be supplied with an output lens to provide a collimated beam, allowing more direct coupling to a microscope or other experimental equipment.
ii) Electronic connection and control
All systems have simple interconnections to allow external interfacing with the Optoscan. For our microprocessor controller based systems this consists of a TTL interface on the rear panel, which takes a standard 37 way D connector. A suitable method of control is from a PC parallel port and a cable. For the PCI card versions of the controller there is a 68 way high density connector on the rear of the Optscan power supply, which should be connected to the PCI card. Windows driver DLLs are available for both the parallel port and PCI card options.
An important feature we provide on our microprocessor controller systems is a "ready" signal. This assists with fast sampling by enabling other equipment (including our photometry modules) to ascertain when the grating and slits have reached their new positions after a wavelength/bandwidth change. This facility seems pretty much essential to us, and we mention it here only because our monochromator appears to be the only one that actually provides such a facility. For further information on how to use the monochromator in conjunction with other equipment, see the section on the microcontroller system.
Also note that the monochromator unit has an external power supply, so that the entire optical system can be sited close to sensitive electrical recording equipment if required, without the risk of introducing hum or other power line interference.