Typical infra-red ccd viewing system combined with multiple emission wavelengths system and field of view defining rectangular diagphram.
We can offer a dedicated CCD camera and monitor system for viewing with infrared illumination while photometric fluorescence measurements are being made. Our standard photomultiplier P240/RBI/001, has negligible sensitivity to infrared light, i.e. wavelengths longer than 700nm, whereas the semiconductor sensors used in CCD cameras still have significant sensitivity out to at least 900nm. Therefore, by illuminating the preparation with light at wavelengths longer than 700nm, it is possible to make camera observations at the same time as fluorescence emissions are being recorded by the photomultipliers at wavelengths within the range of 400 to 600nm.
Under some circumstances, it is necessary to make fluorescence emission measurements at wavelengths in the 600-650nm range. For such measurements, our S20 photomultiplier P240/S20/001 should be used. This has a response extending out to about 800nm, but we have found that acceptable rejection of the CCD viewing light can be achieved by using illumination wavelengths longer than 780nm, as the 700nm dichroic mirror in the emission pathway provides significant additional attenuation of the light reaching the photomultiplier in this wavelength region (for some applications a heat filter is also beneficial). For detection we offer the choice of a compact high quality camera which provides excellent performance for this application, or a high resolution integrating research camera which is more suitable for subsequent upgrade to imaging applications. If you are seeking low light imaging cameras, either intensified or cooled then refer to our imaging pages for current state of the art in this rapidly developing field.