Single Emission Wavelength Systems
Recommended configuration for a single emission output with infra-red CCD monitoring.When using non-ratiometric, or dual excitation dyes such as Fluo-3 and Fura-2 respectively, only a single photodetector is necessary. It is however very useful to be able to define and observe a specific area of interest whilst recordings are being made. To allow this we recommend that a CCD camera is also fitted to the output of the microscope. The configuration shown has a dichroic mirror (typically 600nm long pass) at 45 degrees to the incident light allowing far red and infra-red light to transmit (see section on CCD viewing for more details), whilst reflecting light of lower wavelength to the photomultiplier. Monochrome CCD cameras are by nature highly sensitive to infra-red whereas photomultipliers have little or no sensitivity in this region. The sample can thus be back illuminated with infra-red light allowing the CCD camera to "view" the sample without affecting the visible fluorescence being measured. A rectangular diaphragm is positioned before the splitting mirror so that the the light reaching both the photomultiplier and the viewing camera can be defined.
Required Components:
